Description
Human recombinant HDAC3 (full-length)/NCOR2 (237-489) complex was expressed by baculovirus in Sf9 insect cells using a C-terminal GST-tag for HDAC3 and N-terminal GST-tag for NCOR2. The HDAC3 gene accession, number is NM_003883 and NCOR2 NM_006312.
HDAC3 or Histone deacetylase 3 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex that represses transcription when tethered to a promoter. HDAC3 may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. HDAC3 can also down-regulate p53 function and thus modulate cell growth and apoptosis. Catalytic domain of HDAC4 can interact with HDAC3 via the transcriptional corepressor NCOR2 (1). RELA is a nonhistone substrate of HDAC3 and IKBA-dependent nuclear export of the HDAC3-deacetylated RELA replenishes the depleted cytoplasmic pool of latent NFKB-IKBA complexes (2)